Natural genetic variability reduces recalcitrance in poplar.
Identifieur interne : 001756 ( Main/Exploration ); précédent : 001755; suivant : 001757Natural genetic variability reduces recalcitrance in poplar.
Auteurs : Samarthya Bhagia [États-Unis] ; Wellington Muchero [États-Unis] ; Rajeev Kumar [États-Unis] ; Gerald A. Tuskan [États-Unis] ; Charles E. Wyman [États-Unis]Source :
- Biotechnology for biofuels [ 1754-6834 ] ; 2016.
Abstract
BACKGROUND
Lignin content and structure are known to affect recalcitrance of lignocellulosic biomass to chemical/biochemical conversion. Previously, we identified rare Populus trichocarpa natural variants with significantly reduced lignin content. Because reduced lignin content may lower recalcitrance, 18 rare variants along with 4 comparators, and BESC standard Populus was analyzed for composition of structural carbohydrates and lignin. Sugar yields from these plants were measured at 5 process conditions: one for just enzymatic hydrolysis without pretreatment and four via our combined high-throughput hot water pretreatment and co-hydrolysis (HTPH) technique.
RESULTS
Mean of glucan + xylan yields and the best glucan + xylan yield from rare natural poplar variants were 34 and 50 relative percent higher than the high lignin comparator (BESC-316) at the highest severity HTPH condition, respectively. The ability of HTPH to solubilize a large portion of xylan from solids led to small differences in xylan yields among poplar variants. However, HTPH showed large differences in glucan yields, and hence glucan + xylan yields, among the poplar variants. The high lignin comparator did not display lowest glucan + xylan yields with HTPH at moderate pretreatment severity compared to rare variants, but on the other hand, the low lignin comparator was a consistent top performer at all 5 process conditions. Furthermore, the low lignin comparator (GW-11012) showed a 15 absolute percent increase in glucan + xylan yield compared to the high lignin comparator at the most severe HTPH condition. Overall, relative variant rankings varied greatly with pretreatment severity, but poplar deconstruction was significantly enhanced when the pretreatment temperature was increased from 140 and 160 to 180 °C at the same pretreatment severity factor.
CONCLUSIONS
Glucan yields from high severity HTPH of rare natural poplar variants with reduced lignin content were significantly higher than from the high lignin comparator. Because of the significant effect of processing conditions on the performance rankings, selection of the best performing biofuel feedstocks should be based on sugar yields tested at conditions that represent industrial practice. From a feedstock perspective, the most consistent variants, SKWE-24-2 and GW-11012, provide key insights into the genetic improvement of versatile lignocellulosic biofuels feedstock varieties.
DOI: 10.1186/s13068-016-0521-2
PubMed: 27213013
PubMed Central: PMC4874023
Affiliations:
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Tuskan</name><affiliation wicri:level="2"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge, TN 37831 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Wyman, Charles E" sort="Wyman, Charles E" uniqKey="Wyman C" first="Charles E" last="Wyman">Charles E. Wyman</name><affiliation wicri:level="2"><nlm:affiliation>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, 900 University Ave, Riverside, CA 92521 USA ; Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge, TN 37831 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, 900 University Ave, Riverside, CA 92521 USA ; Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge</wicri:cityArea></affiliation></author></analytic><series><title level="j">Biotechnology for biofuels</title><idno type="ISSN">1754-6834</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Lignin content and structure are known to affect recalcitrance of lignocellulosic biomass to chemical/biochemical conversion. Previously, we identified rare Populus trichocarpa natural variants with significantly reduced lignin content. Because reduced lignin content may lower recalcitrance, 18 rare variants along with 4 comparators, and BESC standard Populus was analyzed for composition of structural carbohydrates and lignin. Sugar yields from these plants were measured at 5 process conditions: one for just enzymatic hydrolysis without pretreatment and four via our combined high-throughput hot water pretreatment and co-hydrolysis (HTPH) technique.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Mean of glucan + xylan yields and the best glucan + xylan yield from rare natural poplar variants were 34 and 50 relative percent higher than the high lignin comparator (BESC-316) at the highest severity HTPH condition, respectively. The ability of HTPH to solubilize a large portion of xylan from solids led to small differences in xylan yields among poplar variants. However, HTPH showed large differences in glucan yields, and hence glucan + xylan yields, among the poplar variants. The high lignin comparator did not display lowest glucan + xylan yields with HTPH at moderate pretreatment severity compared to rare variants, but on the other hand, the low lignin comparator was a consistent top performer at all 5 process conditions. Furthermore, the low lignin comparator (GW-11012) showed a 15 absolute percent increase in glucan + xylan yield compared to the high lignin comparator at the most severe HTPH condition. Overall, relative variant rankings varied greatly with pretreatment severity, but poplar deconstruction was significantly enhanced when the pretreatment temperature was increased from 140 and 160 to 180 °C at the same pretreatment severity factor.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>Glucan yields from high severity HTPH of rare natural poplar variants with reduced lignin content were significantly higher than from the high lignin comparator. Because of the significant effect of processing conditions on the performance rankings, selection of the best performing biofuel feedstocks should be based on sugar yields tested at conditions that represent industrial practice. From a feedstock perspective, the most consistent variants, SKWE-24-2 and GW-11012, provide key insights into the genetic improvement of versatile lignocellulosic biofuels feedstock varieties.</p></div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">27213013</PMID><DateCompleted><Year>2016</Year><Month>05</Month><Day>23</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1754-6834</ISSN><JournalIssue CitedMedium="Print"><Volume>9</Volume><PubDate><Year>2016</Year></PubDate></JournalIssue><Title>Biotechnology for biofuels</Title><ISOAbbreviation>Biotechnol Biofuels</ISOAbbreviation></Journal><ArticleTitle>Natural genetic variability reduces recalcitrance in poplar.</ArticleTitle><Pagination><MedlinePgn>106</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s13068-016-0521-2</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Lignin content and structure are known to affect recalcitrance of lignocellulosic biomass to chemical/biochemical conversion. Previously, we identified rare Populus trichocarpa natural variants with significantly reduced lignin content. Because reduced lignin content may lower recalcitrance, 18 rare variants along with 4 comparators, and BESC standard Populus was analyzed for composition of structural carbohydrates and lignin. Sugar yields from these plants were measured at 5 process conditions: one for just enzymatic hydrolysis without pretreatment and four via our combined high-throughput hot water pretreatment and co-hydrolysis (HTPH) technique.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Mean of glucan + xylan yields and the best glucan + xylan yield from rare natural poplar variants were 34 and 50 relative percent higher than the high lignin comparator (BESC-316) at the highest severity HTPH condition, respectively. The ability of HTPH to solubilize a large portion of xylan from solids led to small differences in xylan yields among poplar variants. However, HTPH showed large differences in glucan yields, and hence glucan + xylan yields, among the poplar variants. The high lignin comparator did not display lowest glucan + xylan yields with HTPH at moderate pretreatment severity compared to rare variants, but on the other hand, the low lignin comparator was a consistent top performer at all 5 process conditions. Furthermore, the low lignin comparator (GW-11012) showed a 15 absolute percent increase in glucan + xylan yield compared to the high lignin comparator at the most severe HTPH condition. Overall, relative variant rankings varied greatly with pretreatment severity, but poplar deconstruction was significantly enhanced when the pretreatment temperature was increased from 140 and 160 to 180 °C at the same pretreatment severity factor.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">Glucan yields from high severity HTPH of rare natural poplar variants with reduced lignin content were significantly higher than from the high lignin comparator. Because of the significant effect of processing conditions on the performance rankings, selection of the best performing biofuel feedstocks should be based on sugar yields tested at conditions that represent industrial practice. From a feedstock perspective, the most consistent variants, SKWE-24-2 and GW-11012, provide key insights into the genetic improvement of versatile lignocellulosic biofuels feedstock varieties.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bhagia</LastName><ForeName>Samarthya</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, 900 University Ave, Riverside, CA 92521 USA ; Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge, TN 37831 USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Muchero</LastName><ForeName>Wellington</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge, TN 37831 USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kumar</LastName><ForeName>Rajeev</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge, TN 37831 USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tuskan</LastName><ForeName>Gerald A</ForeName><Initials>GA</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge, TN 37831 USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wyman</LastName><ForeName>Charles E</ForeName><Initials>CE</Initials><AffiliationInfo><Affiliation>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, 900 University Ave, Riverside, CA 92521 USA ; Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507 USA ; BioEnergy Science Center (BESC), Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge, TN 37831 USA.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>05</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Biotechnol 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